The teenage striker appeared for the Three Lions' senior squad at Euro 2016 but will now feature at youth level, having failed to make an appearance for United so far this season.
Rashford, 18, has been replaced in the United first team by Zlatan Ibrahimovic and therefore new England boss Sam Allardyce has decided to leave him out of his maiden senior squad.
The young marksman burst onto the scene under Louis van Gaal last season, scoring eight times for United.
Rashford earned a place in England's preliminary squad for the Euros and became the youngest Englishman to score on his debut in a 2-1 win over Australia in a warm-up game.
His strike subsequenty earned him a spot on the plane for the European Championships, making his competitive debut when coming on as a substitute in England's 2-1 win over Wales.
Rashford's cameo against Wales meant he became the youngest player to represent England at the Euros since Wayne Rooney in 2004.
He also turned out for his country in the 2-1 defeat at the hands of Iceland in the last 16.
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